Part:BBa_K4743002
NadA+ADH1 terminator with adapter
NadA gene codes for quinolinate synthase, which turns iminoaspartate into quinolate. The whole pathway is shown below:
NAD biosynthesis, iminoaspartate => quinolinate => NAD
Length checking of nadA
We use electrophoresis to check the length of the fragment
Overall metabolic pathway of NMN
NadA is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.
References
1.Fagnocchi, L., Biolchi, A., Ferlicca, F., Boccadifuoco, G., Brunelli, B., Brier, S., Norais, N., Chiarot, E., Bensi, G., Kroll, J. S., Pizza, M., Donnelly, J., Giuliani, M. M., & Delany, I. (2013). Transcriptional regulation of the nadA gene in Neisseria meningitidis impacts the prediction of coverage of a multicomponent meningococcal serogroup B vaccine. Infection and immunity, 81(2), 560–569. https://doi.org/10.1128/IAI.01085-12
2.Michalowski, C. B., Flachmann, R., Löffelhardt, W., & Bohnert, H. J. (1991). Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa. Plant physiology, 95(1), 329–330. https://doi.org/10.1104/pp.95.1.329
3.Christine B. Michalowski, Ralf Flachmann, Wolfgang Löffelhardt, Hans J. Bohnert, Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa , Plant Physiology, Volume 95, Issue 1, January 1991, Pages 329–330, https://doi.org/10.1104/pp.95.1.329
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1284
Illegal BamHI site found at 16 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089
Illegal AgeI site found at 1297 - 1000COMPATIBLE WITH RFC[1000]
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